不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响

发布时间：2018-03-10 07:50

本文选题：环江香猪　切入点：妊娠　出处：《动物营养学报》2017年02期 　论文类型：期刊论文

【摘要】：本试验旨在研究不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响。选用首次妊娠的环江香猪48头,根据体重随机分为2组,每组8个重复(栏),每个重复3头猪。配种后分别饲喂高氮能比饲粮[消化能(DE)为14.73 MJ/kg,粗蛋白质(CP)含量为13.11%,氮能比为0.89]和低氮能比饲粮(DE为12.24 MJ/kg,CP含量为9.77%,氮能比为0.80)。分别于妊娠第45、75和110天每栏取1头母猪,处死屠宰,收集每窝中最大体重、平均体重和最小体重胎猪对应的脐带,利用实时荧光定量PCR方法检测血管发育相关基因——成纤维细胞生长因子(FGF)、血管内皮生长因子(VEGF)、血管内皮生长因子受体2(VEGFR2)和内皮型一氧化氮合酶(e NOS)的表达量。结果表明:与低氮能比饲粮组相比,高氮能比饲粮显著上调了脐带血管发育相关基因的表达量(P0.05),包括妊娠45 d最大体重胎猪的VEGF和VEGFR2表达量,平均体重胎猪的VEGF表达量;妊娠75 d最大体重胎猪的VEGF、VEGFR2和e NOS表达量,平均体重胎猪的VEGF和e NOS表达量,最小体重胎猪的VEGFR2表达量;妊娠110 d最大体重胎猪的FGF、VEGFR2和e NOS表达量,平均体重胎猪的FGF和VEGFR2表达量,最小体重胎猪的VEGF和e NOS表达量。不同妊娠期相同标准体重、同一妊娠期不同标准体重胎猪的脐带血管发育相关基因的表达量均存在差异。由此可见,高氮能比饲粮可能由于其蛋白质和氨基酸水平较高而显著上调了妊娠环江香猪脐带血管发育相关基因的表达量,不同妊娠期或同一妊娠期不同标准体重胎猪的脐带血管发育相关基因的表达量也存在差异。
[Abstract]:The purpose of this study was to study the effects of different nitrogen specific diets on the expression of genes related to umbilical cord blood tube development in pregnant Huanjiang Xiang pigs. 48 first pregnant Huanjiang Xiang pigs were randomly divided into 2 groups according to their body weight. There were 8 replicates in each group. After breeding, high nitrogen energy was 14.73 MJ / kg, crude protein CPR was 13.11, nitrogen energy ratio was 0.89 and low nitrogen energy ratio was 12.24 MJ / kg CP content, nitrogen energy ratio was 9.77%, nitrogen energy ratio was 12.24 MJ / kgCP, nitrogen energy ratio was 14.73 MJ / kg, crude protein / energy ratio was 13.11, and low nitrogen energy ratio was 12.24 MJ / kgCP, respectively. 1 sows per column on the 45th and 110th days of pregnancy, They were killed and slaughtered, and the umbilical cord corresponding to the maximum body weight, average body weight and minimum body weight of each litter were collected. The expression of vascular growth factor-fibroblast growth factor (FGFF), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2) and endothelial nitric oxide synthase (Enos) were detected by real-time fluorescence quantitative PCR. The results showed that: compared with the low nitrogen energy group, Compared with diet, high nitrogen energy significantly upregulated the expression of cord blood vessel development related genes (P0.05), including the expression of VEGF and VEGFR2 in the maximum body weight fetal pigs at 45 days of gestation, and the average VEGF expression in the average body weight fetal pigs, and the VEGFFR2 and e NOS expression in the maximum body weight fetal pigs on the 75th day of gestation, and the expression of VEGFR2 and e NOS in the maximum body weight fetal pigs on the 75th day of gestation. The average expression of VEGF and e NOS, the expression of VEGFR2 in the least weight fetal pigs, the expression of VEGF VEGF FR2 and e NOS in the maximum body weight fetal pigs, and the expression of FGF and VEGFR2 in the average body weight fetal pigs. The expression levels of VEGF and e NOS in minimum weight fetal pigs were different. The expression levels of cord blood vessel development related genes in the same gestational period and the same standard weight fetal pigs were all different. The expression of genes related to the development of umbilical cord blood tube of pregnant Huanjiang Xiang pig was significantly up-regulated by high nitrogen energy compared with diet because of its high level of protein and amino acid. There were also differences in the expression of genes related to the development of umbilical blood vessel in different gestational period or the same gestational period of different standard weight fetal pigs.
【作者单位】：中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术研究中心农业部中南动物营养与饲料科学观测实验站;中国科学院环江喀斯特农业生态实验站香猪研究中心;
【基金】：国家自然科学基金面上项目(31270044,31572421) 中科院“西部之光”人才培养计划重点项目
【分类号】：S828.5

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