有机物不同添加方式对中国黑土有机碳动态及激发效应影响的机制

发布时间：2024-02-13 22:39

　　本研究采用室内模拟培养试验,以中国典型农田土壤吉林黑土为研究对象,探究了葡萄糖不同添加方式(单次和重复添加)对土壤微生物生物量碳氮,土壤二氧化碳释放速率和累积释放量及土壤有机碳激发效应的影响。称取相当于40g干土的鲜样装入250 mL 肖特瓶。每个处理设置5次重复,试验采用两因子完全随机设计。因素(A),包括单次和重复两种不同的葡萄糖添加方式。其中单次添加是指在实验开始时一次添加试验设置的碳添加量,而重复添加是指与单次添加等量的碳分5次添加到培养土壤中,对照处理仅添加等量的蒸馏水。因素(B),包括5个肥力水平依次增加的黑土,分别为:土壤Ⅰ,土壤Ⅱ,土壤Ⅲ,土壤Ⅳ和土壤Ⅴ。¹³C的葡萄糖按土壤有机碳2%的碳添加量,以葡萄糖溶液的形式均匀的加入到培养土壤中。结果表明,不同添加方式对SMBC产生了不同的影响。单次和重复添加处理下SMBC比对照处理分别增加22.9%和11.9%。对土壤肥力水平土壤而言,土壤Ⅳ的SMBC增加最大(比土壤Ⅰ整体上平均增加90%),其次为土壤Ⅴ(比土壤Ⅰ平均增加了 83%)。在培养初期(两周),单次添加处理土壤CO₂释放量...

【文章页数】：69 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
Abbreviation
CHAPTER Ⅰ INTRODUCTION
    1.1 PRIMING EFFECTS
        1.1.1 Factors influencing Priming Effect
        1.1.2 Quantification of Priming Effect
    1.2 SINGLE ADDITION AND REPEATED ADDITION
    1.3 MICROBIAL ACTIVITY
    1.4 MEHANISM OF CARBON STABILITY
    1.5 SOIL ORGANIC CARBON
        1.5.1 SOIL ORGANIC CARBON AND CLIMATE CHANGE SCENARIO
    1.6 SCIENTIFIC QUESTION
    1.7 HYPOTHESIS
    1.8 OBJECTIVES
CHAPTER Ⅱ MATERIALS AND METHODS
    2.1 SOIL
    2.2 BASIC SOIL PROPERTIES
        2.2.1 Soil Organic Carbon
        2.2.2 Total Nitrogen
        2.2.3 Soil pH
        2.2.4 Total Phosphorus
    2.3 EXPERIMENTAL DESIGN
    2.4 MEASUREMENTS
        2.4.1 Analysis of Soil Microbial Biomass Carbon and Soil Microbial biomass Nitrogen
        2.4.2 Procedure
        2.4.3 Analysis of CO2

        2.4.4 Analysis of ¹³CO₂

        2.4.5 Determination of Priming Effect
    2.5 Statistical analysis
CHAPTER Ⅲ SOIL MICROBIAL BIOMASS CARBON AND CARBON DIOXIDE RESPONSE BYGLUCOSE ADDITION IN BLACK SOIL OF CHINA
    3.1 Change in soil microbial biomass carbon (SMBC) by glucose addition patterns
    3.2 Change in soil microbial biomass nitrogen (SMBN) by glucose addition patterns
    3.3 Change in soil carbon dioxide(CO₂) evolved by glucose addition patterns
    3.4 Change in soil total carbon dioxide(CO₂) accumulation by glucase addition patterns
    3.5 CHAPTER SUMMARY
CHAPTER Ⅳ RESPONSE OF　¹³C-CARBON DIOXIDE BY GLUCOSE ADDITION IN BLACKSOIL OF CHINA
    4.1 INTRODUCTION
    4.2 RESULTS
    4.3 CHAPTER SUMMARY
CHAPTER Ⅴ ¹³C- SOIL MICROBIAL CARBON RESPONSE BY GLUCOSE ADDITION INBLACK SOIL OF CHINA
    5.1 INTRODUCTION
    5.2 RESULTS
    5.3 CHAPTER SUMMARY
CHAPTER Ⅵ RESPONSE OF PRIMING EFFECT BY GLUCOSE ADDITION IN BLACK SOIL OFCHINA
    6.1 INTRODUCTION
    6.2 RESULTS
    6.3 CHAPTER SUMMARY
CHAPTER Ⅶ DISCUSSION
    7.1 CHANGE IN SOIL MICROBIAL BIOMASS CARBON (SMBC) BY GLUCOSE ADDITION PATTERNS
    7.2 CHANGE IN SOIL MICROBIAL BIOMASS NITROGEN (SMBN) BY GLUCOSE ADDITION PATTERNS
    7.3 CHANGE IN SOIL CARBON DIOXIDE (CO₂) EVOLVED BY GLUCOSE ADDITION PATTERNS
        7.3.1 CHANGE IN SOIL TOTAL CARBON DIOXIDE (CO₂) ACCUMULATION BY GLUCOSE ADDITION PATTERNS
        7.3.2 CHANGE IN ¹³-CARBON DIOXIDE (CO₂)BY GLUCOSE ADDITION PATTERNS
    7.4 CHANGE IN ¹³C SOIL MICROBIAL BIOMASS CARBON(SMBC) BY GLUCOSE ADDITION PATTERNS
    7.5 CHANGE IN PRIMING EFFECT BY GLUCOSE ADDITION
CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
CURRICULUM VITAE



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