钴影响大麦生长和生理特性的基因型差异及其机制研究

发布时间：2024-02-27 05:39

　　尽管钴(Co2+)在高等植物中为非必需元素,但其对豆科植物有益。然而,植物组织中过量Co2+会对细胞和细胞膜引起不可逆损伤,导致产量、生物量减少、酶失活甚至植株死亡。防止植物中Co2+积累以降低人类饮食中的Co2+含量从而减轻与Co2+相关的健康风险,最经济有效和高效的方法是培育抗Co2+作物或品种。此外,植物中Co2+积累和毒性在不同品种和基因型间差异很大。本研究旨在:(1)明确大麦基因型Co2+毒害耐性的差异;(2)明确大麦Co2+抗耐性的生理机制;(3)明确Co2+在亚细胞水平积累和存在形态的基因型差异。主要结果总结如下:(1)采用水培试验鉴定92个大麦基因型对钴(Co2+)胁迫的耐性。结果显示,与正常条件(无胁迫)比较,Co2+胁迫处理导致生长显著受抑制,Co2+积累增加,但基因型之间差异显著,显示出供试基因型有很广的遗传多样性,Yan66(抗性)和Ea52(敏感)分别受影响最小和最大。(2)以上述鉴定到的3个Co2+胁迫耐性不同的大麦基因型为材料,研究不同Co2+水平(25,50,75和100 μM)对生长、光合参数、Co2+与部分养分元素含量以及氧化胁迫的影响。结果表明,C...

【文章页数】：87 页

【学位级别】：硕士

【文章目录】：
ACKNOWLEDGEMENTS
Abstract
摘要
Abbreviations
Chapter 1 Literature review
    1.1 Background
    1.2 Effects of Co²⁺ on plant growth
    1.3 Effect of Co²⁺ on photosynthetic pigments and hill reaction
    1.4 Effect of Co²⁺ on carbonylated protein accumulation,DNA fragmentation,and induction of DNase activity
    1.5 ROS and modulation of antioxidant enzymes under Co²⁺ stress
    1.6 Effect of Co²⁺ stress on Co²⁺ and some nutrient contents
    1.7 Effect of Co²⁺ stress on Co²⁺ on subcellular distribution and chemical forms of Co2+

    1.8 Research objectives
Chapter 2 Genotypic difference of cobalt stress resistance in barley
    2.1 Materials and Methods
        2.1.1 Plant material and hydroponic culture
        2.1.2 Plant sampling and measurement
        2.1.3 Cobalt content
        2.1.4 Translocation factor of cobalt
        2.1.5 Statistic analysis
    2.2 Results
        2.2.1 Preliminary screening selection
        2.2.2 Second screening experiment
    2.3 Discussion
Chapter 3 The Effect of Co²⁺ Stress on Growth and Physiological Traits and its Association with Co Accumulation in Barley Genotypes Differing in Co²⁺ Resistance
    3.1 Materials and Method
        3.1.1 Plant material and growth condition
        3.1.2 Plant biomass and physiological parameters
        3.1.3 Proline content
        3.1.4 Biochemical analysis
        3.1.5 Lipid peroxidation and reactive oxygen species
        3.1.6 Element content
        3.1.7 Statistical analysis
    3.2 Results
        3.2.1 The effect of Co²⁺ stress on plant growth
        3.2.2 Effects of Co²⁺ stress on chlorophyll content and photosynthetic parameters
        3.2.3 Effects of Co²⁺ stress on the contents of proline,MDA,hydrogen peroxide and superoxide radicals
        3.2.4 Effects of Co²⁺ on total soluble protein (TSP) content
        3.2.5 Effects of Co²⁺ on antioxidant enzyme activities
        3.2.6 Effect of Co²⁺ stress on Co²⁺ and some nutrient contents
    3.3 Discussion
Chapter 4 Subcellular distribution and chemical forms of Co²⁺ in three Barley Genotypes under different Co²⁺ levels
    4.1 Materials and Methods
        4.1.1 Plant material and hydroponic culture
        4.1.2 Treatments and sampling
        4.1.3 Cobalt content
        4.1.4 Separation and measurement of subcellular fractions
        4.1.5 Separation and measurement of chemical forms
        4.1.6 Statistical analysis
    4.2 Results
        4.2.1 The effect of Co²⁺ stress on plant growth and cobalt concentration
        4.2.2 Subcellular distribution of Co²⁺

        4.2.3 Chemical form of Co²⁺

    4.3 Discussion
Chapter 5 Conclusion and prospective
References
List of publications



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