感应交流电延缓nESI中模拟生物样品引发的毛细管堵塞（英文）
发布时间：2021-12-24 10:37
生物样品的检测需要纳升电喷雾离子化质谱（nESI-MS）来适应更苛刻的条件,如高浓度的非挥发性缓冲液和亚微米尺寸的毛细管孔径.以上两个需求会给nESI-MS带来一个棘手的问题:毛细管堵塞.因此,为了延缓毛细管堵塞,我们将感应交流电压施加在尖端内径小于1μm且注入高浓度盐溶液的毛细管上进行电喷雾.结果表明,改进后的毛细管比常规nESI下的毛细管寿命长1～2个数量级.较长的喷雾时间是由于电场方向周期性变化使析出的盐结晶再溶解从而延缓毛细管堵塞.同时,经过长时间（约10 min）高浓度盐溶液的电喷雾,改进后的nESI仍保持稳定和灵敏的信号.最后对模拟细胞外液和细胞内液进行了验证,结果表明感应nESI是一种延缓毛细管堵塞的广泛适用的生物样品分析方法.
【文章来源】：中国科学技术大学学报. 2020,50(11)北大核心CSCD
【文章页数】：8 页
【文章目录】：
1 Introduction
2 Experimental section
3 Results and discussion
3.1 Effects of conventional nESI and induced nESI on the tip clogging
3.2 Dynamic status of tips in conventional and induced nESI
3.3 Investigation of the performance of induced nESI under various conditions
3.4 Versatility of Induced alternating voltage on mitigating the clogging of mimic intracellular fluid and extracellular fluid
4 Conclusions
Conflict of interest
本文编号：3550334
【文章来源】：中国科学技术大学学报. 2020,50(11)北大核心CSCD
【文章页数】：8 页
【文章目录】：
1 Introduction
2 Experimental section
3 Results and discussion
3.1 Effects of conventional nESI and induced nESI on the tip clogging
3.2 Dynamic status of tips in conventional and induced nESI
3.3 Investigation of the performance of induced nESI under various conditions
3.4 Versatility of Induced alternating voltage on mitigating the clogging of mimic intracellular fluid and extracellular fluid
4 Conclusions
Conflict of interest
本文编号：3550334
本文链接：https://www.wllwen.com/projectlw/swxlw/3550334.html
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