乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察.pdf 全文免费在线

  本文关键词：乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察，由笔耕文化传播整理发布。

网友1557281760近日为您收集整理了关于乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察的文档，希望对您的工作和学习有所帮助。以下是文档介绍：·830 · 南方医科大学学报(J South Med Univ) 2012;32(6) 乌司他丁和胸腺肽0c 1联合治疗严重脓毒症的组织病理学观察郭剑颖,邓群,郭旭升,刘双庆,张玉红,何忠杰,林洪远解放军总医院第一附属医院ICU,北京 100048 摘要:目的通过组织病理学观察联合应用乌司他丁和胸腺肽c【1治疗严重脓毒症的效果。方法采用经典大鼠CLP脓毒症模型, 全部动物随机分为对照组、联合治疗组(乌司他丁+胸腺肽Ⅱ1)、乌司他丁治疗组和胸腺肽a1治疗组。制模成功后分别于6、24、48 和72h经尾静脉或皮下注射给药,记录每天死亡数。制模后24、48、72和96h处死动物,留取心、肝、脾、肺、肾、小肠标本,以光镜进行病理学检查,脾脏标本行凋亡检测。结果联合治疗组死亡率显著降低(P=O.0325);组织病理学显示,对照组各脏器损害最严重,单药组其次,联合用药组最轻;此外,对照组脾脏细胞大量凋亡,联合用药组凋亡程度显著下降[(47.4￣10.9)% (39.3士 11.4)%,P=O.0000]。结论乌司他丁和胸腺肽Ⅱ1联合用药可以显著减轻脓毒症大鼠脏器损害,抑制脾脏细胞凋亡,提高脓毒症大鼠存活率。关键词:脓毒症/病理学;乌司他丁;胸腺肽 1;联合用药中图分类号:R631.2 文献标志码:A 文章编号:1673.4254(2012)06.0830.05 doi:10.3969/j.issn.1673-4254.2012.06.016 s/detail/44.1627.R.20120601.1712.006.html Ulinastatin com bined with thym osin al for treatment of severe sepsis: a hist0path0l0gical observation in rats GUO[ianying,DENG Qun,GUO Xusheng,LIU Shuangqing,ZHANG Yuhong,HE Zhongjie,LIN Hongyuan Intensive Care Unit,First Affiliated Hospital ol General Hospital oiPLA Beijing 100048|China Abstract:Objective To evaluate the therapeutic effect of bined with thymosin al in the treatment of severe sepsis in rats.Methods Normal Wistar rats were subject to cecal ligation and puncture(CLP)to establish models of severe sepsis.The rats were then randomized into 4 groups for treatment with saline(contro1),ulinastatin,thymosin al,or bination of the latter two injected throu gh the caudal vein or subcutaqneously at 6,24,48 and 72 h after modeling.The mortality rate was recorded daily and the rats were executed at 24,48,72 and 96 h after CLP to harvest the heart,liver,spleen, lung,kidney and small in testines for pathological examination.The spleen of the rats were taken for detection of apoptosis of the spleen cells.Results Th e mortality rate of the septic rats in bined treatment group was decreased significantly fP= O.0325).The control group showed the most an damage,w_hich was moderate in single drug treatment group and the m ildest bined treatment group.Obvious spleen cell apoptosis was found in the control group,and was significantly ameliorated in bined treatment group【(47.4_+10.9)% VS(39.3-+11.4)%,P=O.0000].bined treatment with ulinastatin and thym osin al can sign ificantly improve the prognosis and an damage and spleen cell apoptosis in rats w ith sever sepsis. Key words:sepsis/pathology;ulinastatin;thymosin bination therapy 严重脓毒症是危重患者面对的最严重威胁之一,其死亡率高、疗效差,也是目前医学领域的重大难题n]。随着研究的不断深人,人们逐渐认识到免疫紊乱是导致脓毒症患者死亡的重要因素。2007年“脓毒症免疫调理治疗协作组”报告,联合采用乌司他丁和胸腺肽a l的免疫调理治疗策略能够明显改善严重脓毒症病人预后,取得该领域治疗研究的重大突破嘲。但由于临床的局限I生, 使该研究缺乏病理学资料,本研究试图通过实验病理学收稿日期:2012—04 18 基金项目:北京市科技计划重大项目作者简介:郭剑颖,主治医师,在读博士研究生,E-maik guojy2003@ yahoo.corn .Cll 通讯作者:邓群,博士,副主任医师,E.mail:vincentdeng036@hotmail. CO m 研究进一步验证抗炎与免疫刺激联合治疗的合理}生。 1材料和方法 1.1实验动物清洁级健康成年雄性 Wistar大鼠 144只,体质量 230~270 g,由军事医学科学院动物中心提供。 1.2主要试剂与仪器大鼠凋亡检测试剂盒 In situ cell death detection Kit(Roche,Germany)。Olympus BX.50光学显微镜(日本Olympus公司),CMIAS系列多功能真彩色(JL京航空航天大学)病理图像分析系统。 1.3 at4-症动物模型制备动物购人后适应性饲养 1周。实验

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  本文关键词：乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察，由笔耕文化传播整理发布。