microdialysis technique Chinese materia medica in vivo analy

  本文关键词：微透析技术在中药成分体内分析研究中的应用，由笔耕文化传播整理发布。

微透析技术在中药成分体内分析研究中的应用

Application of microdialysis technique in in vivo analysis on ingredients of Chinese materia medica

[1] [2] [3] [4]

DONG Ran-ran, WANG Meng, LIU Zhi-dong, ZHU Yan（ 1. Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery

[1]天津中医药大学,天津市现代重点实验室.省部共建国家重点实验室培育基地,现代中药发现与制剂技术教育部工程研究中心,天津300193; [2]天津国际生物医药联合研究院,天津300457

文章摘要：中药及其制剂成分复杂,经过吸收分布后机体内成分的量较低,由于传统的生物样品取样方法存在多种不足,影响中药成分体内过程研究,从而对中药药效物质基础研究造成影响。微透析技术作为一种简便、灵敏的取样技术,在线结合高效液相色谱、质谱等分析技术,可动态揭示中药成分及其代谢产物在机体内的分布变化规律,极大程度提高了中药活性成分体内分析精度,降低实验动物损伤。主要介绍微透析技术的原理、系统组成、特点,重点介绍了其在中药成分体内分析研究中的应用。微透析技术的应用有利于阐明中药药效物质基础及其体内过程,有利于中药靶向给药传递系统的开发,在中药现代化研究中具有广泛的应用前景。

Abstr：Due to the complications of Chinese materia medica（CMM） and its preparations, traditional sampling methods have some limitations and affect the analysis on the ingredients of CMM. The review mainly introduced the principles, system compositions, characteristics of the microdialysis technique according to 70 literatures in the late 10 years, especially its applications on the analysis on CMM in vivo, such as in brain, skin, blood, eyes, intestinal, joint, liver, gallbladder kidney, and lung. Among these organs, brain and skin studies have more experiences. As a sampling method, microdialysis technique can not only reveal the change process of ingredients in CMM, but also can help the development of targeted drug delivery, which has the great value and good perspective in the modernization study on CMM.

文章关键词：

Keyword：:microdialysis technique Chinese materia medica in vivo analysis pharmacodynamic material basis targeted drug delivery system

  本文关键词：微透析技术在中药成分体内分析研究中的应用，由笔耕文化传播整理发布。