新疆非小细胞肺癌患者EGFR基因表达特征及其临床相关性研究
发布时间：2018-10-24 11:08
【摘要】：目的：探讨新疆地区非小细胞肺癌患者中表皮生长因子受体(EGFR)的突变情况及其与临床病理学特征和肿瘤标志物之间的相关性。方法：选取在新疆医科大学第一附属医院行肺癌根治术后非小细胞肺癌患者51例,应用荧光PCR法对肿瘤组织中DNA进行EGFR基因第18、19、20、21等外显子检测,并术前检测血清癌胚抗原等多种肿瘤标志物。结果：EGFR阳性率为49.02%(25/51),其中18号外显子突变率为8%(2/25),19号突变率为52%(13/25),21号突变率为40%(10/25),20号未检测到突变。男性阳性率37.5%(12/32),女性阳性率68.42%(13/19)。女性患者阳性率显著高于男性,有统计学差异(p0.05),腺癌和腺鳞癌与鳞癌比较也有统计学差异(p0.05),而与肿瘤分化程度,淋巴结转移以及TNM分期之间无统计学差异(p0.05)。EGFR基因突变与术前血清癌胚抗原检测值有一定的相关性,有统计学意义(P0.05)。 结论：在新疆女性、肺腺癌患者中EGFR基因突变率为68.42%,为靶向治疗提供有效的依据。术前血清癌胚抗原检测值与EGFR基因突变具有一定的相关性,对无法检测EGFR突变的NSCLC患者能否检测癌胚抗原值来为靶向治疗提供信息需要增加病例及进行相关研究进一步探讨。
[Abstract]:Objective: to investigate the mutation of epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC) in Xinjiang and its correlation with clinicopathological features and tumor markers. Methods: Fifty-one patients with non-small cell lung cancer (NSCLC) after radical resection of lung cancer in the first affiliated Hospital of Xinjiang Medical University were selected to detect the exon of EGFR gene 18m19 / 2021 and other exons by fluorescence PCR assay. Serum carcinoembryonic antigen and other tumor markers were detected before operation. Results: the positive rate of EGFR was 49.02% (25 / 51), the mutation rate of exon 18 was 8% (2 / 25), the mutation rate of 19 was 52% (13 / 25), the mutation rate of 21 was 40% (10 / 25). The positive rate of male was 37.5% (12 / 32) and that of female was 68.42% (13 / 19). The positive rate in female patients was significantly higher than that in men (p0.05). There was also a significant difference between adenocarcinoma and adenosquamous carcinoma and squamous cell carcinoma (p0.05). There was no significant difference between lymph node metastasis and TNM stage (p0. 05). EGFR gene mutation was correlated with serum carcinoembryonic antigen detection value before operation (P0.05). Conclusion: in Xinjiang women, the mutation rate of EGFR gene in lung adenocarcinoma patients is 68.42, which provides an effective basis for targeted therapy. The detection value of serum carcinoembryonic antigen (CEA) was correlated with the mutation of EGFR gene. It is necessary to increase the number of patients with NSCLC who can not detect EGFR mutation to provide information for targeted therapy.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R734.2
本文编号：2291216
[Abstract]:Objective: to investigate the mutation of epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC) in Xinjiang and its correlation with clinicopathological features and tumor markers. Methods: Fifty-one patients with non-small cell lung cancer (NSCLC) after radical resection of lung cancer in the first affiliated Hospital of Xinjiang Medical University were selected to detect the exon of EGFR gene 18m19 / 2021 and other exons by fluorescence PCR assay. Serum carcinoembryonic antigen and other tumor markers were detected before operation. Results: the positive rate of EGFR was 49.02% (25 / 51), the mutation rate of exon 18 was 8% (2 / 25), the mutation rate of 19 was 52% (13 / 25), the mutation rate of 21 was 40% (10 / 25). The positive rate of male was 37.5% (12 / 32) and that of female was 68.42% (13 / 19). The positive rate in female patients was significantly higher than that in men (p0.05). There was also a significant difference between adenocarcinoma and adenosquamous carcinoma and squamous cell carcinoma (p0.05). There was no significant difference between lymph node metastasis and TNM stage (p0. 05). EGFR gene mutation was correlated with serum carcinoembryonic antigen detection value before operation (P0.05). Conclusion: in Xinjiang women, the mutation rate of EGFR gene in lung adenocarcinoma patients is 68.42, which provides an effective basis for targeted therapy. The detection value of serum carcinoembryonic antigen (CEA) was correlated with the mutation of EGFR gene. It is necessary to increase the number of patients with NSCLC who can not detect EGFR mutation to provide information for targeted therapy.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R734.2
【参考文献】
相关期刊论文 前1条
1 黄光辉;杜娟;候襄河;罗教秀;唐渲;;表皮生长因子受体基因突变表达与非小细胞肺癌分化程度关系的研究[J];分子诊断与治疗杂志;2014年02期
，本文编号：2291216
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