蛋白酶体抑制剂MG132调控Nrf2/Keap1-ARE通路对大鼠肠缺血再灌注肠、肺损伤保护作用的研究

发布时间：2021-06-23 06:27

　　目的:研究蛋白酶体抑制剂MG132对大鼠肠缺血再灌注肠、肺损伤的保护作用与调控Nrf2/Keap1-ARE通路之间的关系。方法:将32只健康雄性SD大鼠随机分为4组:正常对照组、I/R组、对照+MG132预处理组、I/R+MG132预处理组。I/R组及I/R+MG132组使用无创动脉夹夹闭肠系膜上动脉（SMA）1小时、再灌注2小时,建立大鼠肠缺血再灌注损伤模型。MG132预处理组于缺血前30分钟腹腔注射0.5 mg/kg MG132,正常对照组仅进行剖腹术及SMA分离术。再灌注结束后,腹主动脉取血并留取肺肠组织样本。光镜下观察大鼠肠、肺石蜡切片组织形态学改变,评价组织损伤程度,检测血清中IL-6、TNF-α水平、26S蛋白酶体活性及组织匀浆中超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）活性,采用免疫组织化学法和免疫印迹法检测肺、肠组织中核转录因子Nrf2（NF-E2-related factor 2）和血红素氧合酶-1（Heme Oxygenase-1,HO-1）蛋白表达。结果:1.与正常对照组相比,I/R组:（1）肺、肠组织有明显的水肿、渗出和炎性细胞浸润（P<0.01,P... 

【文章来源】：大连医科大学辽宁省

【文章页数】：46 页

【学位级别】：硕士

【文章目录】：
中文摘要
英文摘要
前言
材料和方法
结果
讨论
结论
参考文献
综述
    参考文献
附录
致谢


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